Table 2.
Differentially expressed proteins in rice leaves at 48 h post inoculation with Puccinia striiformis f. sp. tritici.
| Spot No. | Identification | GenBank Accession No. | pI/MW (kDa) a | Peptides Count b | Coverage (%) c | Protein Score/Ion Score | Mock Average Qty | Inoculated Average Qty | Fold Change |
|---|---|---|---|---|---|---|---|---|---|
| Up-Regulated Expression | |||||||||
| 4532 | Glyceraldehyde-3-phosphate dehydrogenase b | gi|115450493 | 6.22/47.5 | 14 | 31.8 | 641/747 | 359.7 ± 13.7 | 1349.8 ± 37.4 | 3.76 ± 0.14 |
| 4827 | Transketolase, chloroplast precursor | gi|115466224 | 5.44/74.0 | 25 | 53.1 | 543/773 | 497.3 ± 23.6 | 1267.7 ± 35.4 | 2.55 ± 0.10 |
| 5318 | Chloroplast ATP synthase CF1 alpha chain | gi|20143564 | 5.27/29.4 | 3 | 23.0 | 150/166 | 324.5 ± 10.6 | 732.9 ± 21.7 | 2.26 ± 0.10 |
| 6124 | Ribulose-1,5-bisphophate carboxylase oxygenase | gi|354618517 | 5.80/19.7 | 8 | 38.2 | 268/340 | 729.0 ± 17.0 | 1702.1 ± 31.2 | 2.34 ± 0.08 |
| 6824 | DnaK family protein, chloroplast Hsp70 | gi|125578088 | 5.33/69.8 | 23 | 37.3 | 586/763 | 344.4 ± 24.8 | 1192.8 ± 84.6 | 3.48 ± 0.25 |
| 6826 | OsFtsH1, FtsH protease | gi|115470052 | 5.51/72.9 | 13 | 25.8 | 587/668 | 44.2 ± 2.7 | 335.6 ± 21.2 | 7.59 ± 0.68 |
| 7028 | Carbonic anhydrase | gi|5917783 | 8.41/29.6 | 7 | 41.8 | 203/253 | 451.3 ± 37.2 | 1196.4 ± 79.5 | 2.65 ± 0.25 |
| 7430 | Chloroplast 28 kDa ribonucleo protein | gi|149391365 | 5.03/21.0 | 12 | 75.3 | 234/351 | 316.9 ± 0.3 | 1552.1 ± 13.0 | 4.90 ± 0.06 |
| 7824 | Putative LR10 resistance protein | gi|305691143 | 6.08/105.4 | 8 | 14.7 | 13/37 | 284.7 ± 3.5 | 763.3 ± 17.7 | 2.68 ± 0.13 |
| 8428 | Phosphoribulo kinase | gi|115448091 | 5.68/45.2 | 12 | 25.6 | 153/230 | 210.4 ± 1.3 | 432.7 ± 57.8 | 2.05 ± 0.26 |
| 8722 | Rhodanese-like domain-containing protein chloroplastic-like | gi|115445387 | 5.05/48.4 | 6 | 15.5 | 215/238 | 108.0 ± 1.8 | 650.0 ± 15.9 | 6.03 ± 0.35 |
| 8805 | Chloroplast Hsp70 | gi|15233779 | 5.07/76.6 | 16 | 26.9 | 789/887 | 703.9 ± 13.5 | 2818.1 ± 105.0 | 4.00 ± 0.22 |
| Down-Regulated Expression | |||||||||
| 7718 | T-complex protein | gi|115488160 | 5.12/61.1 | 23 | 38.6 | 683/882 | 2229.3 ± 60.5 | 859.0 ± 35.2 | 0.39 ± 0.01 |
| 8029 | Thioredoxin | gi|297728925 | 8.16/18.9 | 3 | 23.8 | 82/99 | 897.8 ± 22.2 | 275.5 ± 7.4 | 0.31 ± 0.01 |
| 9208 | Ribonucleoprotein chloroplastic-like | gi|149392545 | 4.45/22.2 | 6 | 35.1 | 420/462 | 1228.8 ± 35.4 | 403.5 ± 5.8 | 0.33 ± 0.01 |
a: pI of predicted protein/molecular mass of predicted protein (kDa); b: Number of peptides for protein identification; c: Percentage of protein sequence represented by peptides identified in MS.