Table 2. Effect of new GCR mutator gene defects on the rate of accumulating GCRs in wild-type and pif1-m2 backgrounds.
| Wild type
|
pif1-m2
|
|||
|---|---|---|---|---|
| Relevant genotype | Strain | GCR rate (CANr-5FOAr) | Strain | GCR rate (CANr-5FOAr) |
| Wild type | RDKY3615 | 3.5 × 10−10 (1) | RDKY4343 | 6.3 × 10−8 (180) |
| alo1 Δ | YKJM1921 | 4.7 × 10−8 (134) | YKJM1925 | 1.1 × 10−7 (314) |
| cdc50 Δ | YKJM1379 | 4.8 × 10−9 (14) | YKJM1401 | 2.6 × 10−7 (743) |
| csm2 Δ | YKJM1535 | 2.7 × 10−9 (8) | YKJM1529 | 1.6 × 10−7 (457) |
| elg1 Δ | YKJM1405 | 1.7 × 10−8 (49) | YKJM1403 | 3.0 × 10−7 (857) |
| esc1 Δ | YKJM1479 | 2.3 × 10−9 (7) | YKJM1838 | 1.1 × 10−7 (314) |
| mms4 Δ | YKJM1525 | 5.9 × 10−8 (169) | YKJM1527 | 2.3 × 10−7 (657) |
| rad5 Δ | YKJM1385 | 6.3 × 10−8 (181) | YKJM1387 | 2.2 × 10−7 (633) |
| rad18 Δ | YKJM1389 | 7.1 × 10−8 (202) | YKJM1391 | 2.5 × 10−7 (714) |
| tsa1 Δ | YKJM1467 | 2.6 × 10−9 (7) | YKJM1836 | 3.6 × 10−7 (1,029) |
| ufo1 Δ | YKJM1919 | 2.6 × 10−8 (74) | YKJM1923 | 1.4 × 10−7 (400) |
All strains are isogenic with the wild-type strain RDKY3615 (MATa, ura3-52, leu2Δ1, trp1Δ63, his3Δ200, lys2ΔBgl, hom3-10, ade2Δ1, ade8, hxt13::URA3), with the exception of the indicated mutations. The rate relative to wild type is given in parentheses.