Figure 1.
Effects of oligomycin and celastrol on cell viability. Chemical structures of (A) oligomycin and (B) celastrol were shown; The (C) dose-response and (D) time course of the cell viability for oligomycin and celastrol was determined via MTT assay. After serum starvation, the fully differentiated 3T3-L1 adipocytes were incubated with different doses of oligomycin (5, 10, 20 and 30 µg/mL) and celastrol (5, 10, 20 and 30 μM) in DMSO for 48 h. Time-course dependent (0, 12, 24, 36 and 48 h) was determined for each optimal dose. Effect of oligomycin and celastrol on nuclear factor kappa B (NF-κB) expression was performed via (E) immunoblotting analysis. β-actin was used as loading control. Protein levels measured were normalized against β-actin. Results were expressed as means ± SEM (n = 6–8). * p < 0.05 vs. untreated control.