Fig. 3.
Global fitting of simulated by the bimolecular model time courses by the induced fit and preequilibrium of two conformers models. The simulated time courses were produced by adding 1 RU amplitude of uniform noise to the major component resolved by the two-species model (k1 = 7.0·103 M-1·s-1and k-1 = 0.20 s-1). The simulated time curves (dotted lines) were globally fitted with following parameters of the induced-fit model (Eq. 1) k12 = 1 × 106M-1·s-1, k21 = 3 × 103 s-1, k23 = 20.7 s-1, k32 = 0.2 s-1,(Left, smooth lines) or the model assuming the existence of two conformers of the binding site in the unbound TCR (Eq. 2) k12 = 7.4 × 102 M-1·s-1, k21 = 1.2 × 105 s-1, k23 = 1 × 106M-1·s-1, k32 = 0.2 s-1 (Right, smooth lines).