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. 2014 Dec 9;15(12):22772–22785. doi: 10.3390/ijms151222772

Figure 3.

Figure 3

Assays to characterize the type of cell death performed at different times after photodynamic treatments. (A) HeLa cells incubated with 1 µM ZnPc for 1 and 3 h, processed for the Annexin V/propidium iodide (ANV/PI) assays immediately after irradiation and observed in fluorescence microscopy under blue and green excitation light (overlay); (B) HeLa cells subjected to the same treatments, processed for TUNEL assay 3 h later and observed in fluorescence microscopy under blue excitation; (C) HeLa cells incubated with 1 µM ZnPc (1 and 3 h), processed for indirect immunofluorescence for cyt-c immediately after irradiation, counterstained with H-33258 and observed in fluorescence microscopy under UV and blue excitation light (merged image). Scale bar: 20 µm.