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Stability of 2′-O-methyl-modified EGS R1 and R2 in the presence of human plasma and HeLa nuclear extracts. EGSs were either loaded directly on polyacrylamide denaturing gels (-, lanes 1 and 4) or incubated in either DMEM containing 10% human plasma (Plasma, lanes 2 and 5) or in HeLa nuclear extracts (Promega) (HeLa, lanes 3 and 6) at 37°C for 24 h before loading on the gels.
