Fig. 3.

Cleavage of ³²P-labeled substrate rta-S by human RNase P in the presence of different EGSs. No RNase P was added to the reaction mixture in lane 1. We incubated 5 nM of Rta-S substrate alone (lanes 1-2) or in the presence of 5 nM R1 (lane 3) or R2 (lane 4) in the presence of 2 units of RNase P. Cleavage reactions were carried out in buffer A (50 mM Tris, pH 7.0/100 mM NH₄Cl/10 mM MgCl₂) at 37°C for 1 h.