Fig. 6.
Expression of KSHV proteins in EGS-treated cells. We first treated 1 × 105 BCBL-1 cells with liposome complexes containing EGSs, isolated them by using FACS analysis, and then incubated them either in the absence (lanes 1, 5, 9, and 13) or the presence (lanes 2-4, 6-8, 10-12, and 14-16) of 20 ng/ml TPA, and we finally harvested them at either 24 (A and B)or72h(C and D) after TPA induction. Protein samples were isolated from cells treated with liposome complexes in the absence of EGSs (-, lanes 1-2, 5-6, 9-10, and 13-14) or in the presence of R1 (lanes 3, 7, 11, and 15) and R2 (lanes 4, 8, 12, and 16). The samples were separated by SDS/PAGE, transferred to membranes, and reacted with antibodies against human actin (A), KSHV Rta (B), ORF59 (C), and ORF65 proteins (D).