Figure 6.

Competition between GluTR and EF-Tu for Glu-tRNA^Glu. The deacylation of Glu-tRNA₂^Glu catalyzed by GluTR (0.5 µM) in presence of EF-Tu is shown. Deacylation was monitored when GluTR was untreated (▲,♦) or previously treated (□,◊) with 300 μM H₂O₂ and in the presence of EF-Tu, treated (◊) or untreated (♦) with 300 μM H₂O₂. The deacylation of Glu-tRNA₂^Glu in the absence of GluTR or EF-Tu was used as a control (●). Each curve is the mean of three experimental replications. For each point, the standard deviation was no higher than 15%.