Figure 2.

Phosphorylation level of PSII proteins increased by heat treatment. (a) Thylakoid membrane proteins were subjected to SDS-PAGE and subsequent immunoblotting using specific phospho-threonine antibodies; (b) Identification of the phosphorylated thylakoid proteins. Purified phosphorylated thylakoid proteins in L40 that were purified using the ProQ Diamond Phosphoprotein Enrichment Kit were separated by SDS-PAGE, and the bands in the dotted boxes were detected by peptide MS finger printing analysis. Mascot search results are shown in Supplementary Table S1; and (c) Thylakoid membrane proteins, stacked grana and unstacked stroma lamellae were subjected to SDS-PAGE and subsequent immunoblotting using specific phospho-threonine antibodies.