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PSI–LHCII supercomplex increased by heat stress in light. Thylakoid membrane proteins were solubilized with 0.1% n-dodecyl-α-d-maltoside (α-DM) and 1% digitonin and subjected to Blue Native-Polyacrylamide Gel Electrophoresis (BN-PAGE) and subsequent immunoblotting using specific phospho-threonine antibodies.
