Figure 1.

The Edc4 interaction with mTORC1: (A) CCRF-CEM cells were lysed, and Edc4 or raptor containing component of mTORC1 were co-immunoprecipitated using Edc4 and raptor antibody respectively. Immunoblotting with indicated antibodies confirmed co-precipitation of Edc4 with raptor and vice versa. No bands were detected in mock control (beads and whole cell lysates without adding antibody); and (B) CCRF-CEM cells were transiently transfected with myc-tag raptor pRK5 plasmid. After transfection, the cells were lysed and myc-tag raptor component of mTORC1 was specifically immunoprecipitated with myc-tag monoclonal antibody. The myc-tag IP elute was separated on SDS-PAGE and immunoblotted with corresponding antibodies which showed substantial association of Edc4 with the myc-tag raptor component (Western blot: WB, represents total cell lysates: TCLs).