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Edc4 interaction with mTORC1, but not with mTORC2: CCRF-CEM cells were lysed and endogenous mTOR complexes were immunopurified using raptor and rictor antibodies, respectively. The IP elutes were resolved on SDS-PAGE and immunoblotted with indicated antibodies which confirmed that Edc4 was only associated with mTORC1 but not with mTORC2.
