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. 2014 Dec 12;15(12):23179–23195. doi: 10.3390/ijms151223179

Figure 3.

Figure 3

Edc4 co-localization with raptor component of mTORC1: CCRF-CEM cells were grown, fixed, permeabilized and both rabbit anti-Edc4 and mouse anti-raptor were added and incubated overnight. Fluorescent signals were detected using labeled anti-mouse Cydye3 and anti-rabbit Alexa Fluor 488. Raptor and Edc4 co-localization in CCRF-CEM cells was analyzed using an Axiovert 200M confocal microscope and processed with Image J software. The yellow overlay images demonstrate the high co-localization of raptor (red) with Edc4 (green) in processing bodies (P bodies, arrows indicate P bodies). The scatter plot of the individual pixels was obtained from two source images. Quantitative co-localization analysis illustrates increased co-localized pixels of Edc4 with raptor. At least 30 cells were observed per experiment (Scale bars = 5 μm) and experiments were repeated five times (only two experimental replicates A and B are shown). DAPI (4,6-diamidino-2-phenylindole) blue colour indicates cell nucleus.