Figure 4.

Leucine starvation and rapamycin treatment increased Edc4 protein expression: CCRF-CEM cells were grown, treated and lysed as described in methods section. Immunoblotting with Edc4 antibody detected significant change in the Edc4 expression in leucine starved and rapamycin treated cells as compared to control (without starvation). β tubulin was used as a loading control. The representative data present mean ± SEM of at least five independent experiments and the significance was determined by Student’s t-test (* p < 0.05; ns: Non-significant).