Figure 5.

Edc4 and raptor interaction is rapamycin sensitive and rapamycin reduced Edc4 phosphorylation of serine residues: (A) CCRF-CEM cells were treated and lysed as described in the methods section. Endogenous mTORC1 was specifically immunoprecipitated using raptor antibody and resolved on the gel. Immunoblotting with corresponding antibodies detected decreased Edc4 signal in the treated as compared to non-treated controls (n = 4); and (B) CCRF-CEM cells were treated and lysed. Edc4 was specifically immunoprecipitated using Edc4 antibody and the IP elute was resolved on SDS-PAGE. Immunoblot analysis with phosphoserine antibody detected decreased phosphorylation signal in the rapamycin treated samples as compared to DMSO. The representative data are mean ± SEM of at least four independent experiments and the significance was determined by Student’s t-test (* p < 0.05).