Figure 7.

Rapamycin enhanced mRNA decapping activity: CCRF-CEM cells were treated with either rapamycin or vehicle control for one hour and (A) Total RNA was extracted from the cells, while the capped mRNAs were specifically isolated from total RNA using terminator exonuclease and lithium chloride precipitation. The removal of 18S and 28S rRNA from total 5'-capped mRNA was confirmed by 1.5% agarose gel electrophoresis; (B) After confirmation of 5'-capped mRNA purity, the total 5'-capped mRNA was run on the microchip gel and quantified; and (C) Bar diagram representation of five independent experiments (mean ± SEM), where significance was determined by Student’s t-test (** p < 0.005).