Fig. 3.

Fus1 and Fus2 activate NF-κB through a RelB/p50 transcriptional dimer. Induction of NF-κB DNA activity was observed in nuclear extracts of Fus1 and Fus2 stably expressing cell lines compared with the vector. The identity of protein components of the NF-κB DNA-binding activity was determined by electrophoretic mobility-shift assay with antibodies to p50, p52 (not shown), RelA (not shown), RelB, and c-Rel (not shown). RelB and p50 generated shifted signal with both fusions. Competition experiments were carried out with mutant and wild-type NF-κB oligonucleotides in 20× excess. The data shown are representative of three independent experiments.