Table 1.
Primers used for gene clone and verification.
| Primer Name | Primer Sequences (5'–3') | Amplicon Size (bp) | Purpose |
|---|---|---|---|
| hsp21.4DP-F | ATGGARGAAGAAATGASAARTT | 241 | Intermediate fragment amplification |
| hsp21.4DP-R | TCGACHGTCTTVACRACGAT | ||
| hsp20.6DP-F | CCTMGCCGYCTGDTGGAYCARC | 467 | |
| hsp20.6DP-R | TCCTTGATCTCCTTGCGVACGG | ||
| hsp19.6DP-F | AAGTBAACCTDGACGTGCAGCATT | 296 | |
| hsp19.6DP-R | TTTCACCTCCTTGCGCACTGGT | ||
| hsp21.4RACE-5' | CTTCAATGACTTGCCGTCGCCCTC | 418 | Rapid-amplification of cDNA ends (RACE) |
| hsp21.4RACE-3' | AGCACAGTGACAGCAGACAGTTGGC | 1062 | |
| hsp20.6RACE-5' | ATTCCACGGACTCGGGTTCCACGC | 510 | |
| hsp20.6RACE-3' | TTGCTGCTGGACCTTTGCTGACGA | 610 | |
| hsp19.6RACE-5' | TCTGGTAAAGCGTAGCGGCGGGT | 502 | |
| hsp19.6RACE-3' | GGCAGTTTACCCGCCGCTACGCT | 327 | |
| hsp21.4cDNA-F | ATGGGGAGTACTGCCTTG | 963 | Verification of open reading frame (ORF) |
| hsp21.4cDNA-R | TGCCTGAATACATCCCTTA | ||
| hsp20.6cDNA-F | AAGATGTCTCTGTTGCCA | 559 | |
| hsp20.6cDNA-R | CACTTTACTTCTTTTCCTTT | ||
| hsp19.6cDNA-F | GTGCGAAACAAGTACAAAGC | 692 | |
| hsp19.6cDNA-R | CAAAGAGAACACTGAAAGGAAT | ||
| hsp21.4DNA1-F | TGTCTGCTGTAGAGTGCGTAG | 372 | Verification of genome |
| hsp21.4DNA1-R | TTGAACTCGCCCCTGATC | ||
| hsp21.4DNA2-F | TGGGACAGCTTGAACTCG | 1529 | |
| hsp21.4DNA2-R | AGTGCTTCTGGATGGGGA | ||
| hsp21.4DNA3-F | CGACAGGAACATCCCCATCCAGAA | 697 | |
| hsp21.4DNA3-R | TATTGACATCTAAAACATTCGTAAC | ||
| hsp20.6DNA-F | AAGATGTCTCTGTTGCCA | 559 | |
| hsp20.6DNA-R | CACTTTACTTCTTTTCCTTT | ||
| hsp19.6DNA-F | GTGCGAAACAAGTACAAAGC | 692 | |
| hsp19.6DNA-R | CAAAGAGAACACTGAAAGGAAT |