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. 2014 Dec 15;15(12):23269–23282. doi: 10.3390/ijms151223269

Figure 1.

Figure 1

(A) 2D-immunoblotting obtained by probing D-MS-CSF Pool I with the corresponding UD-MS-CSF Pool I (UD, undepleted); An equivalent protein pattern was obtained for MS-CSF Pools II, III and IV; (B) 2D-immunoblotting obtained by probing D-MS-CSF Pool II with the corresponding UD-MS-CSF Pool II; (C) 2D-immunoblotting obtained by probing D-MS-CSF Pool III with the corresponding UD-MS-CSF Pool III; (D) silver-stained 2D proteomic map of D-MS-CSF Pool I separated in pH range 4–9. Immunoreactive spots (numbered from 1 to 8) were identified as Tf isoforms by matching immunoblots in (A–C) with this reference map. The correspondence was established by using albumin isoforms as anchors for matching (see the Experimental Section); (E) 2D-immunoblotting obtained by probing Tf purified from human blood with a commercial anti-transferrin antibody; (F) 2D-immunoblotting obtained by probing Tf purified from human blood with UD-MS-CSF Pool III; (G) Magnification of 2D-immunoblotting reported in (E); this image represents the control condition of the competition assay; and (H) 2D-immunoblotting obtained by probing purified human Tf with UD-MS-CSF Pool III pre-incubated for five hours with 200 μg/mL of purified Tf. OMD: Orosomucoid-1, synonymous Alpha-1-acid glycoprotein 1; APOJ: Apolipoprotein J, synonymous Clusterin; APOA-1: Apolipoprotein A-I.