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(A) 2D-immunoblotting obtained by probing D-MS-CSF Pool IV with the corresponding MS-Se (Se, serum) pool; (B) 2D-immunoblotting obtained by probing D-MS-CSF Pool IV with one of the sera used in the MS-Se pool (see Table S1); and (C) 2D-immunoblotting obtained by probing Tf purified from human blood with the same serum as in (B). Albumin was used as an anchor for matching with the reference CSF map (see the Experimental Section).
