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The effect of thapsigargin on [Ca2+]cyt measured in unfertilized egg cells incubated in calcium-free IVF medium. Thapsigargin was added to unfertilized eggs at: 0.1 μM (a), 1 μM (b), 10 μM (c) and at 50 μM (d). The arrows designate the times of the addition of the drug.
