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. 2014 Dec 19;15(12):23836–23850. doi: 10.3390/ijms151223836

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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The distance between donor and acceptor fluorophores changes as a function of denaturant concentration and reveals conformational changes during apoflavodoxin (un)folding. The Förster transfer distance (R₀; open squares) changes as a function of denaturant concentration in a folding state-dependent manner. Calculated distances (r_da; black dots) between donor Alexa Fluor 488 and acceptor Alexa Fluor 568 track the features of apoflavodoxin (un)folding. Values of R₀ are obtained by using parameters as described in Supplementary Information SI3. Values of r_da are obtained by using Equation (7).