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. 2014 Dec 22;15(12):23851–23877. doi: 10.3390/ijms151223851

Table 2.

Applications of CE in the determination of proteins in transgenic cultivars.

Cultivar GMO Analyte CE Mode Detection CE Conditions Sample Treatment Ref.
Maize Bt resistant (Bt11) Zein fraction CZE UV BGE: 100 mM phosphate buffer pH 3 containing 60% v:v ethanol; polyacrylamide coated capillary, 40 cm × 50 µm; voltage, 20 kV; temperature, 25 °C; injection, 0.5 psi × 10 s Extraction with 70% v:v ethanol with 2% v:v β-mercaptoethanol [38]
Maize Bt resistant (Bt11) Albumin and globulin fraction CZE UV BGE: 100 mM phosphate buffer pH 3 containing 60% v:v ethanol; capillary, 40 cm × 50 µm; voltage, 20 kV; temperature, 25 °C; injection, 0.5 psi × 10 s Albumin fraction: extraction with water and ultrafiltration (3 KDa cut off). Globulin fraction: extraction with 50 mM Tris buffer (pH 7.8) containing 50 mM KCl and 5 mM EDTA and ultrafiltration (3 KDa cut off) [38]
Maize Bt resistant (Bt11) Protein water/ACN (75/25, v/v) fractions EKC UV BGE: 80 mM phosphate buffer pH 2.5 containing 5% ACN and 0.01% DAB dendrimer; capillary, 40 cm × 50 µm; voltage, 10 kV; temperature, 25 °C; injection, 3.4 KPa × 5 s Extraction with water/ACN (75:25, v:v) +s0.3% acetic acid [39]
Maize Bt resistant Water soluble protein fraction CZE UV BGE: 100 mM H3PO4, 50 mM Tris pH 2.25 or 200 mM iminodiacetic acid pH 2.26: capillary, 20 cm × 50 µm; voltage, 10 kV; temperature, r.t.; injection, 1.2 KPa × 22 s Extraction with water [40]
Maize Bt resistant Trypsin digested water soluble protein fraction CZE UV BGE: 200 mM iminodiacetic acid pH 2.26; capillary, 20 cm × 50 µm; voltage, 10 kV; temperature, r.t.; injection, 12 mbar × 12 s Extraction with water and trypsin digestion [41]
Soybean Glyphosate resistant (SB10) Trypsin digested water/ACN (80/20, v/v) protein fractions CZE ESI-MS (+) BGE: 0.5 M formic acid; capillary, 90 cm × 50 µm; voltage, 25 kV; temperature, 25 °C; injection, 0.5 psi × 20 s Extraction with water/ACN (80:20, v:v) and trypsin digestion [42]

r.t.: room temperature.