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. 2014 Dec 17;5:5804. doi: 10.1038/ncomms6804

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(a) Full-length untreated, HOCl-treated and MCA-treated RidA was analysed in an OrbiTrap Elite mass spectrometer. Up to seven chlorine atoms (and five oxygen atoms) are added to RidA. (b) Time-resolved analysis of MCA-mediated modification of RidA-C107S. Samples for mass spectrometry were taken directly after addition of MCA and after time points indicated. After 30 min of incubation, DTT reduction was carried out for 30 min before mass spectrometry.