Skip to main content
. 2004 Jun 3;101(24):9127–9132. doi: 10.1073/pnas.0401845101

Fig. 2.

Fig. 2.

Dissection of the drug-responsive fragments leads to definition of ADRES elements. (A) The 2,146-bp hA6 fragment was cut with ApaI to yield three smaller fragment of 262, 1,437, and 447 bp, respectively. Fragments were introduced into the pGL3tk vector and tested in LMH cells for induction by vehicle, PB, or PIA, as described. The responsive 1,437-bp fragment was further digested with BglII and BstEII, yielding fragments of 795, 198, and 597 bp. The 1,089-bp fragment spanning from the BstEII site to the 3′ end of the hA6 fragment showed no response to drugs (data not shown). Data represent the mean of at least three independent experiments plus one SD. (B) The hA8 fragment was cut at SacI and NsiI restriction sites, and the resulting fragments of 100 and 818 bp, as well as subfragments of the 818-bp piece (347, 240, and 231 bp), were cloned into the pGL3tk reporter vector. The ability of these fragments to mediate induction in LMH cells was tested as described. Data represent the mean of at least three independent experiments plus one SD.