Fig. 5.

The ADRES elements mediate drug induction in a larger genomic context. A reporter construct harboring the 795- and 818-bp drug-responsive elements (see Fig. 2 A and B) directly upstream of the 234-bp hALAS1 promoter was tested for induction in LMH cells with vehicle (0.1% DMSO), 400 μM PB, 250 μM PIA, 400 μM metyrapone (Met), 10 μM clotrimazole (Clo), or 10 μM mifepristone (RU486). By comparing reporter activities with activities obtained with a reporter construct containing only the 234-bp hALAS1 promoter, the fold increase of expression caused by the two ADRES is shown. Data represent the mean of four independent experiments plus one SD. ^*, P < 0.05; ^**, P < 0.01.