Figure 8.
Decreased autophagy in Nrf2 deficient mice during S. aureus 5×106 peritonitis. A) HO-1 in Nrf2 KO mice show an overall diminished response to sepsis. At 24h Nrf2 KO mice had significantly lower HO-1 levels than WT mice at 24h in the same sepsis model. B) LC3-II is significantly decreased in Nrf2 deficient mice at 24h compared to wild-type C57BL/6 mice. C) p62 levels show accumulation at 24h at the protein level significantly compared to wild-type mice at 24h. D) p62 mRNA shows increased transcription compared to 0h wild-type and Nrf2 deficient mice although decreased copy number compared to wild-type mice at 24h. E) Western blots of above proteins. (* = p < 0.05 compared to WT 24h)