Figure 4.

Decreased protein expression of Nefh and Cplx1 in mice lacking PGC-1α. Western blot analysis was performed on cerebellar homogenates of PGC-1α^+/+ and ^−/− littermates at 30 days of age to determine if protein of PGC-1α-dependent genes was downregulated in a similar manner as the transcript. Expression of Cplx1 and Nefh, but not Pacsin2, was significantly reduced in ^−/− compared to ^+/+ animals (A). Representative Western blots are shown in (B). One-tailed t-tests. ^*p < 0.05. n = 7/group. Data are presented as mean ± SEM.