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. 2015 Jan 6;8:441. doi: 10.3389/fncel.2014.00441

Figure 7.

Figure 7

PGC-1α ablation results in decreased Purkinje cell viability and function by 6 weeks of life. (A) Stereological counts of Purkinje cells stained with hematoxylin and eosin (H&E) were conducted to obtain an unbiased estimate of the number of Purkinje cells. At 6 weeks of age, PGC-1α−/− had significantly fewer Purkinje cells than +/+ littermates. (B) Representative pictures of H&E stained Purkinje cells from 6-week-old animals. Note the deep staining in +/+ animals compared to the often undetectable stain in the Purkinje cell layer (PCL) of −/− animals. GCL, granule cell layer. ML, molecular layer. Scale bar = 100 μm. (C) Loose patch electrophysiology was conducted in acute cerebellar slices to determine if loss of PGC-1α leads to Purkinje cell dysfunction. (D) Purkinje cell spike rate was significantly reduced in PGC-1α−/− compared to +/+ mice. Representative traces shown in (C) Two-tailed t-tests. **p < 0.005, *p < 0.05. n/group indicated on bar histograms. For electrophysiology data, the number of animals is indicated in parentheses after the number of cells. Data presented as mean ± SEM.