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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Immunol. 2014 Sep 3;193(7):3726–3735. doi: 10.4049/jimmunol.1400210

FIGURE 4.

FIGURE 4

Aspartic protease is required for N-terminal product-mediated C-terminal product degradation. RAW macrophages expressing recombinant Myc-tagged C-terminal fragment transduced a retrovirus expressing TM-GFP or Nterm-TM-GFP, incubated for 6 h with DMSO, chloroquine (5 μM), or MG132 (10 μM) (A), or for 12 h with z-FA-fmk (10 μM) or pepstatin A (1 μM) (B); proteins were analyzed with anti-Myc, anti-GFP, or anti–β-actin by immunoblot. Data are representative of three independent experiments.