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Correlation between neutrophil influx and total surface area of deposited nanoparticles. Doses of 0, 18, 54, or 162 µg/mouse carbon black or TiO2 nanoparticles were deposited in lungs of mice by intratracheal instillation and the number of neutrophils in bronchial lavage fluid was determined as described previously.74 N = 6 per group, except for vehicle controls where n = 22: (a) 24 h after instillation and (b) 28 days post exposure.
