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. 2014 Jul 30;93(5):928–943. doi: 10.1111/mmi.12707

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© 2014 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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A transcription start site in the esxA-esaA intergenic region of strain RN6390. DNA sequence covering the promoter region and transcriptional start site of the esxA gene through to the start of esaA. Note that the sequence of this region is identical between all strains used in this study. Transcriptional start points (indicated TSP1 and TSP2), mapped by 5′-RACE, are indicated in bold. TSP1 was identified from strains RN6390 and USA300, whereas TSP2 was identified from RN6390 only. Putative −35/−10 regions are underlined, the coding regions for esxA and esaA are shown in upper case letters and boxed (esxA is truncated to save space), and putative Shine–Dalgarno sequences are given in italics. A predicted rho-independent terminator for the esxA gene is indicated above the sequence.