Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| S. aureus | ||
| RN6390 | NCTC8325 derivative, rbsU, tcaR, cured of φ11, φ12, φ13 | Novick et al. (1993) |
| COL | MRSA, agr | Dyke et al. (1966), Gill et al. (2005) |
| USA300 | Community-acquired MRSA | McDougal et al. (2003) |
| Newman | ATCC 25904 | Duthie and Lorenz (1952), Baba et al. (2008) |
| SA113 | NCTC8325 derivative, agr, rbsU, tcaR, hsdR | Iordanescu and Surdeanu (1976) |
| RN6390 ess | Complete deletion from esxA – SAOUHSC_00269 | This work |
| RN6390 esxA | esxA deletion | This work |
| RN6390 esaA | esaA deletion | This work |
| RN6390 essA | essA deletion | This work |
| RN6390 esaB | esaB deletion | This work |
| RN6390 essB | essB deletion | This work |
| RN6390 essC | essC deletion | This work |
| RN6390 esxC | esxC deletion | This work |
| RN6390 esxB | esxB deletion | This work |
| COL ess | Complete deletion from Sacol0271 (esxA) -Sacol0282 | This work |
| SA113 ess | Complete deletion from esxA – SAOUHSC_00269 | This work |
| E. coli | ||
| JM110 | rpsL thr leu thi lacY galK galT ara tonA tsx dam dcm glnV44 Δ(lac-proAB) e14-[F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK−mK+) | Stratagene |
| MC1061 | F− Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ− e14− mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2(r−m+) | Casadaban and Cohen (1980) |
| DC10B | dam+ dcm− hsdRMS endA1 recA1 | Monk et al. (2012) |
| Plasmids | ||
| pBluescript KS+ | General purpose E. coli cloning vector, ampr | Stratagene |
| pMAD | E. coli/S. aureus shuttle vector, temperature sensitive, ampr, eryr | Arnaud et al. (2004) |
| pIMAY | E. coli/S. aureus shuttle vector, temperature sensitive, cmlr | Monk et al. (2012) |
| pRMC2 | E. coli/S. aureus shuttle vector, inducible protein expression, ampr, cmlr | Corrigan and Foster (2009) |
| pRMC2h | pRMC2 variant coding for N-terminal hexahistidine tag | This work |
| pEsaA-nhis | pRMC2h expressing His6-EsaA | This work |