Strains |
|
|
S. aureus |
|
|
RN6390 |
NCTC8325 derivative, rbsU, tcaR, cured of φ11, φ12, φ13 |
Novick et al. (1993) |
COL |
MRSA, agr
|
Dyke et al. (1966), Gill et al. (2005)
|
USA300 |
Community-acquired MRSA |
McDougal et al. (2003) |
Newman |
ATCC 25904 |
Duthie and Lorenz (1952), Baba et al. (2008)
|
SA113 |
NCTC8325 derivative, agr, rbsU, tcaR, hsdR
|
Iordanescu and Surdeanu (1976) |
RN6390 ess |
Complete deletion from esxA – SAOUHSC_00269
|
This work |
RN6390 esxA |
esxA deletion |
This work |
RN6390 esaA |
esaA deletion |
This work |
RN6390 essA |
essA deletion |
This work |
RN6390 esaB |
esaB deletion |
This work |
RN6390 essB |
essB deletion |
This work |
RN6390 essC |
essC deletion |
This work |
RN6390 esxC |
esxC deletion |
This work |
RN6390 esxB |
esxB deletion |
This work |
COL ess |
Complete deletion from Sacol0271 (esxA) -Sacol0282
|
This work |
SA113 ess |
Complete deletion from esxA – SAOUHSC_00269
|
This work |
E. coli |
|
|
JM110 |
rpsL thr leu thi lacY galK galT ara tonA tsx dam dcm glnV44 Δ(lac-proAB) e14-[F' traD36 proAB+
lacIq
lacZΔM15] hsdR17(rK−mK+) |
Stratagene |
MC1061 |
F− Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ−
e14−
mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2(r−m+) |
Casadaban and Cohen (1980) |
DC10B |
dam+ dcm− hsdRMS endA1 recA1 |
Monk et al. (2012) |
Plasmids |
|
|
pBluescript KS+
|
General purpose E. coli cloning vector, ampr
|
Stratagene |
pMAD |
E. coli/S. aureus shuttle vector, temperature sensitive, ampr, eryr
|
Arnaud et al. (2004) |
pIMAY |
E. coli/S. aureus shuttle vector, temperature sensitive, cmlr
|
Monk et al. (2012) |
pRMC2 |
E. coli/S. aureus shuttle vector, inducible protein expression, ampr, cmlr
|
Corrigan and Foster (2009) |
pRMC2h |
pRMC2 variant coding for N-terminal hexahistidine tag |
This work |
pEsaA-nhis |
pRMC2h expressing His6-EsaA |
This work |