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. 2014 Nov 12;138(1):94–109. doi: 10.1093/brain/awu310

Figure 8.

Figure 8

Absence of neuronal apoptosis, deposits of complement, and lymphocytic infiltrates in the hippocampus of mice infused with patients’ CSF. (A and B) TUNEL and cleaved caspase 3 immunolabelling of a representative area of CA3 (area with maximal IgG binding and lower NMDAR concentration) of an animal infused with patients’ CSF, showing lack of apoptotic cells. A section of the same region in an animal with transient middle cerebral artery occlusion (stroke model) shows apoptotic cells in the penumbra (left). (C) Same CA3 region as in (A) immunostained for C5b-9 showing lack of deposit of complement. A section of the same region in the indicated stroke model shows presence of complement in the penumbra (left). (D and E) Same CA3 region as in (A) immunostained for T (CD3) and B (CD45R) lymphocytes showing absence of inflammatory infiltrates. A section of spleen was used as control tissue showing the presence of CD3 (green) and CD45R (red) cells. Scale bar = 10 µm. Total number of animals examined: patients’ CSF n = 5; control CSF n = 5. Scale bars = 20 µm.