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Trace of FM4-64 via endocytosis and AtGALT31A-localized small compartments. Images were taken at 10 min (A, D), 40 min (B, E) and 140 min (C, F) after application of FM4-64 to AtGALT31A-mCer3 or SYP61-mCer3 expressed N. benthamiana leaves. Both AtGALT31A-mCer3-localized compartments (A–C, green) and SYP61-mCer3-localized TGN subdomains (D–F, green) were not colocalized with the FM4-64-internalized early endosome and its aggregates (magenta) up to 140 min. Scale bars = 10 µm.
