The effect of Wortmannin treatment. AtGALT31A-mCer3 (A–D), SYP61-mCer3 (E–H) and ST-CFP (I–L) (green) expressed N. benthamiana leaves was cotreated with Wortmannin and FM4-64 (magenta) for 0 min (A, E, I), 20 min (B, F, J), 40 min (C, G, K) and 100 min (D, H, L). Merger of the AtGALT31A compartments and SYP61-localized TGN to FM4-64 compartments appeared at 100 min (D and H), while the Golgi stack defined by ST-CFP was not merged to FM4-64 compartments at 100 min (L). Scale bars = 10 µm.