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. 2014 Aug 5;93(5):975–991. doi: 10.1111/mmi.12713

Figure 5.

Figure 5

Localization of GFP–SirA in vivo.

A. Schematic diagram showing the modified sirA locus used for localization studies. Chromosomal sirA was replaced with gfp–sirA under the control of its native expression system.

B. GFP–SirA localization during sporulation of B. subtilis 150 min post resuspension in starvation media. Membrane dye FM5-95 was used to highlight the outlines of the cells. Scale bar = 3 μm. gfp–sirA (NR3).

C. Temporal analysis of GFP–SirA foci formation during sporulation. At least 500 cells were analysed at each time-point.

D. Colocalization of GFP–SirA with oriC during sporulation (150 min post resuspension in starvation media). More than 200 cells were analysed and a representative image is shown. Scale bar = 3 μm. gfp–sirA oriCtetO/TetR−mCherry (NR164).

E. Colocalization of GFP–SirA with the replisome during sporulation (150 min post resuspension in starvation media). More than 100 cells were analysed and a representative image is shown. Scale bar = 3 μm. gfp–sirA dnaN–mCherry (NR168).