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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Bone. 2014 Nov 22;72:81–91. doi: 10.1016/j.bone.2014.11.010

Figure 5. pCREB binding sites correlate with PTH-regulated genes and are located within epigenetically delineated enhancers.

Figure 5

A) 17496 forskolin-induced pCREB binding sites were associated with 11993 genes as assessed using the GREAT algorithm. ≥ 1208 of the 2677 genes associated with the PTH-regulated transcriptome in IDG-SW3 osteocytes (24 hr at 100nM PTH vs vehicle, 2 fold, 95% confidence interval, moderated t test). contained pCREB. B) ChIP-seq tag density tracks (normalized to 107 tags) for pCREB binding in MC3T3-E1 preosteoblast and for selected histone modifications in IDG-SW3 osteoblasts (red), IDG-SW3 osteocytes (blue with overlap in purple), MC3T3-E1 preosteoblasts (yellow) and MC3T3-E1 preosteoblasts (blue with overlap in green) at the gene loci Id3 (B), Bcl3 (C), Mef2d (D) and Kdm4a (E) loci.