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. Author manuscript; available in PMC: 2015 Jan 6.
Published in final edited form as: Gastroenterology. 2007 May 21;133(2):647–658. doi: 10.1053/j.gastro.2007.05.022

Figure 2.

Figure 2

miR-21 is up-regulated in human primary HCC. Total RNA was isolated from HCC (T) and matching control (N), and Northern blot analysis was performed as described in the Materials and Methods section. The signal in each lane was quantified by Kodak Imaging software and the ratio of miR-21 to 5S ribosomal RNA was determined. Asterisks denote human primary HCC in which mir-21 is up-regulated. In the first 9 samples the signal is higher because more RNA (20 μg) was loaded. For samples 10–20, 10 μg of RNA was loaded.