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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: Cell Rep. 2014 Aug 21;8(5):1545–1557. doi: 10.1016/j.celrep.2014.07.049

Figure 6. Nuclear PACS-2 regulates the p53-SIRT1 interaction.

Figure 6

(A) Total p53 was immunoprecipitated from HCT116 cells and Ac-p53 (K382) analyzed by western blot. (B) Endogenous SIRT1 was immunoprecipitated from HCT116 WT or p53−/− cells treated with 0.5 µM Dox (6hr) as indicated and co-precipitating p53 analyzed by western blot. (C) HCT116 cells were treated with 0.5 µM Dox (14hr), endogenous SIRT1 immunoprecipitated and co-precipitating p53 analyzed by western blot. p53 was quantified using AlphaView (Protein Simple).