TABLE 2.
Primers and siRNAs used in this study
| Primer name | Direction of primera | Sequence |
|---|---|---|
| qGAPDH F | F | GCACAGTCAAGGCCGAGAAT |
| qGAPDH R | R | GCCTTCTCCATGGTGGTGAA |
| qMHC F | F | TCCAAACCGTCTCTGCACTGTT |
| qMHC R | R | AGCGTACAAAGTGTGGGTGTGT |
| qMYOD F | F | CATCCGCTACATCGAAGGTC |
| qMYOD R | R | GTGGAGATGCGCTCCACTAT |
| qMYOGENIN F | F | AGCGCAGGCTCAAGAAAGTGAATG |
| qMYOGENIN R | R | CTGTAGGCGCTCAATGTACTGGAT |
| q human ACTIN β F | F | G GCACCAGATCATGTTTGAG |
| q human ACTIN β R | R | GAGTCCATCACGATGCCAGT |
| q human MHC F | F | CTTCCCTGCACCAGATTCTC |
| q human MHC R | R | GTATAAGCCCGAGGTGGTGA |
| q human MYOD F | F | GGGGCTAGGTTCAGCTTTCT |
| q human MYOD R | R | GCTCTGGCAAAGCAACTCTT |
| q human MYOGENIN F | F | GCCAGACTATCCCCTTCCTC |
| q human MYOGENIN R | R | GAGGCCGCGTTATGATAAAA |
| q human DRR F | F | CTGGGCAGAGCAGCCAAGGGAGCTG |
| q human DRR R | R | GAGGGGCTCATTTGGTGGGGAGTGGG |
| CER ChIP F | F | GGGCATTTATGGGTCTTCCT |
| CER ChIP R | R | CTCATGCCTGGTGTTTAGGG |
| DRR ChIP F | F | TCAGGCCAGGACCATGTCT |
| DRR ChIP R | R | CTGGACCTGTGGCCTCTTAC |
| Myogenin promoter ChIP F | F | GAATCACATGTAATCCACTGGA |
| Myogenin promoter ChIP R | R | ACGCCAACTGCTGGGTGCCA |
| 1F | F | AGCCTCAGGATGAGCTGTGT |
| 1R | R | CTCAATGCAGGGCCTCTTAG |
| 2F | F | TTCCAAAAAGGAGGAAGCAA |
| 2R | R | ATGGATGTGGGGTTCATCAT |
| Nested 5′ RACE primer outer | R | TCTCTCCCATTGGTCGGTTG |
| Nested 5′ RACE primer inner (R-B′) | R | GTTATTCACCGAGGGACACG |
| F-A | F | TAGCCAAGGGAGCTGAAATG |
| R-B | R | GTTATTCACCGAGGGACACG |
| siMUNC-1 | GGAUGAGCUGUGUGCUUCUTT | |
| siMUNC-2 | CGACCAAUGGGAGAGAGCATT | |
| si HUMAN MUNC | AGUCCUCCCCUCUCAGCUCCCtt | |
| si-MYOD | CCAAUGCGAUUUAUCAGGUGCUUUG | |
| pLPCX-MUNC F (in fusion cloning primer) | F | GCCTCGGCCAAACATCGATA |
| pLPCX-MUNC R (in fusion cloning primer) | R | GAGTCCGGTAGCGCTAGC |
| MUNC GT110AG F (MUNC splicing mutant cloning primer) | F | GAGCTGTGTGCTTCTCCAGAGCAGTGGGCCTACAGCCTAAG |
| MUNC GT110AG R (MUNC splicing mutant cloning primer) | R | CTTAGGCTGTAGGCCCACTGCTCTGGAGAAGCACACAGCTC |
a
F, forward; R, reverse.