FIG 6.

DJ_H transcription is strongly decreased on the excluded alleles. (A) A scheme showing a partially (DJ_H2) rearranged IgH locus and the resulting DJ_H transcript (Dμ) and a fully (V_HDJ_H2) rearranged gene and the resulting VDJ-Cμ transcript. (B) B220⁺, AA4.1-positive (AA4.1⁺), and IgM^b-positive (IgM^b+) B cells from the bone marrow of hemizygous mice with the indicated genotypes were sorted (B220⁺, AA4.1⁺, and IgM^a-positive [IgM^a+] B cells were used for B1-8/+ mice). The WT allele is derived from C57BL/6 mice. Total RNA was extracted and reverse transcribed. Spliced DJ_H transcripts were quantified by qPCR. WT transcript levels were set as 100% of the signal. Gapdh and Ywhaz transcripts were used for normalization. The histograms show the standard errors (n = 3). ***, P < 0.001. (C) RNA from sorted homozygous pro-B cells was assayed as described in the legend to panel B (n = 3). ***, P < 0.001. (D) RNA from sorted homozygous pro-B cells was assayed as described in the legend to panel B (n = 3). ***, P < 0.001; **, P < 0.01.