FIG 4.

Regulation and stability of CTSB mRNA. (A) Primary human macrophages were incubated for 8 or 48 h under hypoxia followed by Pol II-ChIP at the CTSB promoter. (B) Pol II-ChIP samples were used to probe different sections of the CTSB gene for Pol II binding. Enrichment is shown as the percentage of input. (C) Primary human macrophages were incubated under hypoxic conditions for 4 or 44 h and treated with actinomycin D (ActD; 2.5 μg/ml) for an additional 4 h. DMSO-treated controls were set to 1. CTSB mRNA was analyzed by qPCR and normalized to 18S rRNA. Data are expressed as means ± SEM (n ≥ 3 experiments). *, P < 0.05.