TABLE 3.

Sequences of primers used for methylation analysis

P0 region	Forward (5′ . . . 3′)	Reverse (5′ . . . 3′)	Size (bp)
1	TTTTTGTAGTTAATATTTTTTTTTTGGGGTTTTGGTTG	CRTACTATAACCAAACTTAACTACCCTC	350
2	GTTTTTYGGGGGTTGGTTTAAGGTTTTTTTTTGTAG	TCCTCCCCCTCRACTTTTTAATCTAAAAATCCC	332
3	GGTTTTGTYGTTTGGGGGAGGGGGTTAG	AAAACAACRACTAATCCCCAAATCCC	292
4	ATTTAGATTTTTATATTTTGTATAGGTTGTGGTTTG	TACACCCCCAACCAAAACCCCAAAAAAAAAATATTAAC	349
5	TGGGTAGAGGYGAATTTTTGGATTTAAGTTTAGG	ACCCTACRAAATACACCCCCAACCAAAACC	295
6	TGATTTGTAATTTTTAGTTTTTTTTTTTATTTTTGGG	ACCCTACRAAATACACCCCCAACCAAAACC	254
7	AATTTTYGGGGGGTGGGGAGGTTTG	AACCTAACTCRAAAAAAAACTATCCCCTCTC	166
8	AATTTYGGTTTTTTGTTTTGAGTTTATGAGG	ACACRAAAAAACTAAAACCCAAAAATAAAAAAAAAAAC	339
9	AGGAAGGGTYGGGGATAGGTTGTGTATTAGAGTTTTTG	ACACRAAAAAACTAAAACCCAAAAATAAAAAAAAAAAC	300