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. 2014 Oct 15;29(1):61–69. doi: 10.1096/fj.14-261867

Figure 3.

Figure 3.

Acetaldehyde elicits release of ATP, β-HEX, and renin in HMC-1 cells. A) Incubation of HMC-1 with acetaldehyde for 20 min elicits a concentration-dependent release of endogenous ATP (n = 6–12). Basal ATP release was 374.3 ± 51.08 nmol/L (n = 11). B) Expression of CD39 in MC modulates acetaldehyde-induced release of β-HEX and renin. WT HMC-1 and HMC-1 infected with empty vector, CD39-overexpressing vector, lentivirus carrying sh scrambled, and sh-silenced CD39 sequence were incubated with acetaldehyde (300 μM) for 20 min in the absence or presence of A740003 (P2X7R blocker; 3 μM, 15 min preincubation); β-HEX and renin content was measured in the supernatants at the end of incubation. Basal β-HEX release was 2.28 ± 0.32% (n = 21), and basal renin release (angiotensin I formed) was 5.16 ± 1.07 ng/h/mg protein (n = 16). Bars are mean ± sem (n = 5–16). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. WT cells, respectively, by unpaired t test.