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. 2014 Oct 17;29(1):208–215. doi: 10.1096/fj.14-259663

Figure 3.

Figure 3.

Lentiviral KD of DEN-1 in T84 cells. A) Lentiviral shRNA-mediated KD of DEN-1 in T84 intestinal epithelial cells results in diminished DEN-1 mRNA relative to shNTC. B) DEN-1 KD results in loss of DEN-1 protein in cytoplasmic fraction and Cul-2 neddylation in nuclear fraction (n = 3 independent experiments). C) Western blotting of T84 shNTC and DEN-1 KD cells after increasing doses of MLN4924 for 1 h demonstrating increased HIF-1α stabilization in nuclear fraction (n = 3). D) Relative mRNA expression of HIF targets Glut and Eno-1 in shNTC and DEN-1 KD cells after 2 h treatment with 10 μM AKB4924 (n = 2). *P < 0.05, as determined by Student’s t test. Error bars represent sem.