Figure 1.
Immunofluorescence images of ∆lmxgt1-3 null mutant parasites expressing LmxGT1-GFP (A) or LmxGT2-GFP (B) from an episomal expression vector. Parasites were inoculated at a density of 1 × 105 cells/ml and grown for 6 d in RPMI 1640 medium containing high (10 mM) or low (0.5 mM) initial glucose. Samples were withdrawn at days 2, 3, 4, 5, and 6 (D2–D6) for fixation and imaging by deconvolution microscopy. Promastigotes were stained with anti-α-tubulin and Alexa Fluor 594-conjugated antibodies (red) to visualize the subpellicular microtubule network. Green represents GFP.