Table 1. Method verification measures: linearity, accuracy, precision and verification HFCS sugars fraction.
| HFCS sugars fraction | Method linearitya R2 | Method accuracyb mean % recovery (95% CI) | Method precisionc mean % recovery (RSD, %) (95% CI) | Method verificationd mean % difference (95% CI) |
|---|---|---|---|---|
| Composite data | ||||
| Fructose | 0.9998 | 100.77 (99.06; 102.47) | 100.43 (0.85) (99.03; 101.83) | 0.010 (−0.067; 0.086) |
| Glucose | 0.9989 | 101.13 (99.61; 102.64) | 101.68 (0.85) (101.13; 102.23) | 0.142 (0.078; 0.205) |
| DP2+ | 0.9987 | 103.33 (102.41; 104.25) | 104.07 (0.85) (103.78; 104.35) | −0.138 (−0.170; −0.105) |
| Lab 1 | ||||
| Fructose | 0.9999 | 99.40 (98.05; 100.75) | 98.83 (0.49) (98.52; 99.14) | −0.075 (−0.211; 0.061) |
| Glucose | 0.9999 | 100.73 (95.78; 105.67) | 101.05 (0.55) (100.68; 101.42) | 0.213 (0.106; 0.320) |
| DP2+ | 0.9995 | 103.06 (100.19; 105.94) | 104.48 (8.20) (99.07; 109.73) | −0.119 (−0.186; −0.053) |
| Lab 2 | ||||
| Fructose | 0.9998 | 102.13 (100.04; 104.23) | 102.04 (0.50) (101.63; 102.44) | 0.093 (0.027; 0.160) |
| Glucose | 0.9980 | 101.53 (99.36; 103.68) | 102.31 (0.60) (101.86; 102.76) | 0.070 (0.006; 0.134) |
| DP2+ | 0.9979 | 103.59 (102.08; 105.09) | 103.75 (0.59) (103.30; 104.18) | −0.156 (−0.159; −0.153) |
Abbreviations: CI, confidence interval; RSD, relative standard deviation.
R2 is the coefficient of determination.
Calculated by straight line fit of aqueous standards over a range of concentrations (80, 100 and 120% of their expected concentration in commercial beverages).
Calculated by the average % recovery of standards in beverage matrix over a range of concentrations (80, 100 and 120% of their expected concentration in commercial beverages); 10 replicates per concentration were performed. 95% CI: mean % recovery±1.96*s.e.
Calculated by the average % recovery at concentration of 100% 10 replicates.
Calculated by the average of the differences between actual % sugar and % sugar of the authentic standard; 10 replicates at concentration of 100%. The actual % sugar is the value reported by the lab using 10 replicates. 95% CI: average difference ±1.96*s.e.