Skip to main content
. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Biochem J. 2013 Oct 1;455(1):95–106. doi: 10.1042/BJ20121755

Figure 1. Identification of ZO-2 as a potential SNX27 PDZ binding partner.

Figure 1

(A) mpkCCD cell extracts (lanes i and iii) or lysis buffer (lane ii) were incubated with GST–SNX27 PDZ (lanes ii and iii) or GST controls (lane i). Bound proteins were separated by SDS/PAGE and identified by Coomassie Blue staining. Prominent bands between 28 and 40 kDa are GST fusions. Band marked by arrow was excised, in-gel digested with trypsin and identified as ZO-2 by MS. Molecular mass is shown on the left-hand side in kDa. (B) ZO-2 peptide coverage statistics.